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产品描述：人α-烯醇化酶(ENO1/ENO1L1/MBPB1/MPB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、血浆样本中的ENO1含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于生命科学领域研究，如疾病分子机制探索、细胞代谢调控分析、肿瘤微环境研究等方向，为科研人员提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2 phospho D glycerate hydro lyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Alpha enolase ELISA Kit; Alpha enolase like 1 ELISA Kit; Alpha-enolase ELISA Kit; C myc promoter binding protein ELISA Kit; C-myc promoter-binding protein ELISA Kit; EC 4.2.1.11 ELISA Kit; eno1 ELISA Kit; ENO1L1 ELISA Kit; ENOA_HUMAN ELISA Kit; Enolase 1 (alpha) ELISA Kit; Enolase 1 (alpha) like 1 ELISA Kit; Enolase 1 ELISA Kit; Enolase alpha ELISA Kit; MBP 1 ELISA Kit; MBP-1 ELISA Kit; MBP1 ELISA Kit; MBPB1 ELISA Kit; MPB 1 ELISA Kit; MPB-1 ELISA Kit; MPB1 ELISA Kit; MYC promoter binding protein 1 ELISA Kit; NNE ELISA Kit; Non neural enolase ELISA Kit; Non-neural enolase ELISA Kit; Phosphopyruvate hydratase ELISA Kit; Plasminogen binding protein ELISA Kit; Plasminogen-binding protein ELISA Kit; PPH ELISA Kit; Tau crystallin ELISA Kit
缩写：ENO1
Uniprot No.：P06733
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENO1/ENO1L1/MBPB1/MPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-105 | |||||
回收率：
| The recovery of human ENO1/ENO1L1/MBPB1/MPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-100 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3212.3192.3202.225101.9821.9671.9751.88051.4701.5761.5231.4282.51.0030.9950.9990.9041.250.6380.6520.6450.5500.6250.3800.3910.3860.2910.3120.2930.2990.2960.20100.0930.0970.095 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ENO-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled ENO-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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