| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HHM, PLP, BDE2, PTHR, PTHRP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PTHLH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TBC1D4抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索获取)：
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1. **文献名称**: *"Insulin-regulated trafficking of the TBC1D4/AS160 Rab GTPase-activating protein in 3T3-L1 adipocytes"*
**作者**: Sano H, Eguez L, Teruel MN, et al.
**摘要**: 研究通过特异性抗体检测TBC1D4在脂肪细胞中的亚细胞定位及胰岛素刺激下的磷酸化变化，揭示其通过调控Rab蛋白活性促进GLUT4囊泡转运的分子机制。
2. **文献名称**: *"Development of phospho-specific antibodies to evaluate TBC1D4 phosphorylation in human skeletal muscle"*
**作者**: Treebak JT, Birk JB, Hansen BF, et al.
**摘要**: 描述了针对TBC1D4关键磷酸化位点(如Ser318/Ser588)抗体的开发与验证，并应用于人体骨骼肌样本，证明其在胰岛素抵抗与运动干预研究中的有效性。
3. **文献名称**: *"AS160/TBC1D4 deficiency enhances peripheral glucose uptake in mice through increased expression of GLUT1"*
**作者**: Fritsche L, Lehmann R, Schleicher ED, et al.
**摘要**: 利用TBC1D4敲除小鼠模型及Western blot抗体分析，发现TBC1D4缺失通过上调GLUT1表达改善外周组织葡萄糖摄取，提示其在代谢疾病中的潜在治疗靶点。
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建议通过PubMed或Google Scholar搜索具体文献，关键词可尝试 **"TBC1D4 antibody"**、**"AS160 phosphorylation"** 或结合研究领域如 **"insulin signaling TBC1D4"**。
**Background of TBC1D4 Antibody**
TBC1D4 (Tre-2/Bub2/Cdc16 domain family member 4), also known as AS160 (Akt substrate of 160 kDa), is a Rab GTPase-activating protein (Rab GAP) involved in insulin-regulated glucose transport. It plays a critical role in the translocation of glucose transporter GLUT4 to the plasma membrane in response to insulin signaling. TBC1D4 contains multiple phosphorylation sites, including key residues phosphorylated by Akt/PKB (e.g., Thr642), which inactivate its Rab GAP activity, allowing Rab proteins (e.g., Rab10) to promote vesicle trafficking and GLUT4 mobilization.
Antibodies targeting TBC1D4 are widely used in research to study insulin signaling, glucose metabolism, and metabolic disorders like diabetes and insulin resistance. They enable detection of TBC1D4 expression, phosphorylation status, and interactions in tissues or cell lines (e.g., skeletal muscle, adipose tissue). Such antibodies are essential tools for Western blotting, immunofluorescence, and immunoprecipitation assays. Dysregulation of TBC1D4 function has been linked to impaired glucose uptake, making it a potential therapeutic target. Researchers also utilize these antibodies to explore TBC1D4’s role in other cellular processes, including vesicle trafficking and autophagy.
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