首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经特异性烯醇化酶(NSE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的ENO2含量。ENO2作为靶点，它是烯醇化酶的一种。ENO2在神经组织及神经内分泌来源的细胞中特异性表达，在肿瘤发生发展等过程可能发挥重要作用。其研究机制主要围绕探究其在细胞代谢、增殖、凋亡等方面的分子调控作用，以寻找潜在治疗靶点和干预手段。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于神经损伤模型评估、神经细胞分化研究、神经相关疾病机制探索以及药物干预效果监测等科研领域，为神经科学基础研究提供稳定可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Eno2 ELISA Kit; Eno-2Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
缩写：ENO2
Uniprot No.：P17183
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 84-97 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of mouse NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 92-108 | ||||
| EDTA plasma (n=4) | 91 | 84-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.6222.5422.5822.475502.2092.1672.1882.081251.4631.4871.4751.36812.50.7620.7340.7480.6416.250.3580.3330.3460.2393.120.2520.2450.2490.1421.560.1800.1680.1740.06700.1060.1080.107 | ||||||
货期：3-5 working days
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