首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠烯醇酶1(ENO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENO1含量。ENO1即烯醇化酶1，是糖酵解途径中的关键酶。研究发现，它在肿瘤细胞中高表达，与肿瘤的生长、侵袭和转移相关。其机制可能是通过调节糖代谢，为肿瘤细胞提供能量，同时影响肿瘤微环境等，有望成为肿瘤治疗的新靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外定量分析小鼠模型或细胞实验中ENO1的动态表达变化，可为代谢通路研究、肿瘤生长微环境分析、炎症反应机制探索等基础科研领域提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Eno1 ELISA Kit; Eno-1 ELISA Kit; Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
缩写：ENO1
Uniprot No.：P17182
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 81-90 | ||||||
| 1:4 | Average % | 104 | |||||
| Range % | 100-107 | ||||||
| 1:8 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
回收率：
| The recovery of mouse ENO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 107 | 103-110 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.5572.6012.5792.41620002.2912.1862.2392.07610001.5531.5621.5581.3955000.9810.9980.9900.8272500.5820.5710.5770.4141250.4140.4320.4230.26062.50.2950.2900.2930.13000.1610.1640.163 | |||||||
货期：3-5 working days
×
