首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠外核苷酸焦磷酸酶/磷酸二酯酶家族成员1(ENPP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENPP1含量。ENPP1 即 ectonucleotide pyrophosphatase/phosphodiesterase 1，是一种外切核苷酸酶。其与多种生理病理过程相关，如骨代谢、血管钙化等。研究机制方面，它能水解 ATP 等产生焦磷酸，调控细胞外核苷酸信号传导，也是癌症等疾病潜在治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本产品适用于基础科研领域，包括但不限于探究ENPP1在代谢综合征、血管钙化或肿瘤微环境中的分子机制，以及构建相关疾病动物模型后的生物标志物动态监测。支持研究者通过血清、血浆或组织样本进行跨维度的ENPP1表达水平比较分析，为深入解析其生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Enpp1 ELISA kit; Npps ELISA kit; Pc1 ELISA kit; Pdnp1Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA kit; E-NPP 1 ELISA kit; Lymphocyte antigen 41 ELISA kit; Ly-41 ELISA kit; Phosphodiesterase I/nucleotide pyrophosphatase 1 ELISA kit; Plasma-cell membrane glycoprotein PC-1) [Cleaved into: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA kit; secreted form] [Includes: Alkaline phosphodiesterase I ELISA kit; EC 3.1.4.1); Nucleotide pyrophosphatase ELISA kit; NPPase ELISA kit; EC 3.6.1.9 ELISA kit; Nucleotide diphosphatase)] ELISA kit
缩写：ENPP1
Uniprot No.：P06802
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:10 | Average % | 90 | |||||
| Range % | 82-99 | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 87-101 | ||||||
| 1:40 | Average % | 95 | |||||
| Range % | 85-104 | ||||||
回收率：
| The recovery of mouse ENPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 88-99 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.5912.5752.5832.489101.4651.4921.4791.38550.7860.8020.7940.7002.50.4240.4380.4310.3371.250.2720.2740.2730.1790.6250.1680.1720.1700.0760.3120.1270.1290.1280.03400.0920.0950.094 | |||||||
货期：3-5 working days
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