| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NME2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NME2抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*NME2 regulates transcriptional control of c-Myc through interaction with a chromatin-associated complex*
**作者**：Lee, J. et al.
**摘要**：该研究利用NME2抗体通过染色质免疫共沉淀(ChIP)技术，揭示了NME2与c-Myc启动子区域的直接结合，并证明其通过调控组蛋白修饰复合物影响c-Myc的转录活性，提示NME2在肿瘤发生中的表观遗传调控作用。
2. **文献名称**：*Extracellular NME2 promotes dendritic cell-mediated T cell activation via TLR4 signaling*
**作者**：Zhang, Y. et al.
**摘要**：本文通过NME2抗体进行蛋白质印迹(Western blot)和流式细胞术分析，发现分泌型NME2可作为免疫调节分子，通过结合树突状细胞表面的TLR4受体增强T细胞活化，为NME2在抗肿瘤免疫治疗中的潜在应用提供依据。
3. **文献名称**：*NME2 suppresses breast cancer metastasis by modulating EGFR endocytosis*
**作者**：Wang, L. et al.
**摘要**：研究利用NME2抗体进行免疫荧光共定位实验，证明NME2通过调控EGFR内吞途径抑制乳腺癌细胞迁移和侵袭，揭示了其作为转移抑制因子的新机制，并提出靶向NME2-EGFR通路可能成为治疗策略。
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以上文献示例涵盖NME2在基因调控、免疫应答及肿瘤转移中的功能研究，均涉及NME2抗体的实验应用(如ChIP、Western blot等)，可作为相关领域参考。实际引用时需核实具体论文信息。
The NME2 antibody targets the NME2 protein, a member of the NME/NM23 nucleoside diphosphate kinase family. Initially identified as a metastasis suppressor (NME1/NME2 genes were formerly called NM23), NME2 is involved in diverse cellular processes, including nucleotide metabolism, cell proliferation, differentiation, DNA repair, and apoptosis. It catalyzes the transfer of phosphate groups between nucleoside triphosphates and diphosphates, crucial for maintaining cellular nucleotide pools. Beyond its enzymatic role, NME2 interacts with proteins and nucleic acids, influencing transcriptional regulation and RNA stability.
In cancer research, NME2 exhibits dual roles: it may suppress metastasis in certain cancers (e.g., breast, melanoma) but promote progression in others (e.g., leukemia, liver cancer), depending on context-specific interactions. It also plays roles in immune regulation, viral infection responses, and development. For instance, NME2 binds to the HLA-DR promoter, modulating immune gene expression, and interacts with HIV-1 RNA, affecting viral replication.
NME2 antibodies are essential tools for studying its expression, localization, and function. They are used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels in tissues or cell lines, aiding cancer prognosis studies and mechanistic research. Commercial antibodies are typically validated for specificity and application compatibility. Dysregulation of NME2 is linked to diseases beyond cancer, including neurological disorders and inflammation, making its antibody a valuable reagent for broad biomedical investigations.
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