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产品描述：人膜外核苷酸焦磷酸酶/磷酸二酯酶家族成员2(ENPP2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENPP2含量。ENPP2是备受关注的靶点。它参与多种生理病理过程，与炎症、肿瘤等疾病相关。研究机制上，其编码的自分泌运动因子具有核苷酸酶和溶血磷脂酶D活性，可产生溶血磷脂酸，影响细胞增殖、迁移等，在疾病治疗研究中潜力大。试剂盒检测范围为25 ng/mL-2000 ng/mL，适用于体外科研实验中检测ENPP2的蛋白表达变化，可广泛应用于肿瘤生物学、代谢调控机制、心血管疾病及免疫微环境等基础研究领域，为探索ENPP2在疾病发生发展中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATX ELISA Kit; ATX X ELISA Kit; Autotaxin ELISA Kit; Autotaxin t ELISA Kit; E NPP 2 ELISA Kit; E-NPP 2 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase 2 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 ELISA Kit; Enpp2 ELISA Kit; ENPP2_HUMAN ELISA Kit; Extracellular lysophospholipase D ELISA Kit; FLJ26803 ELISA Kit; LysoPLD ELISA Kit; NPP2 ELISA Kit; PD IALPHA ELISA Kit; PDNP2 ELISA Kit; Phosphodiesterase I alpha ELISA Kit; Phosphodiesterase I/nucleotide pyrophosphatase 2 ELISA Kit; Plasma lysophospholipase D ELISA Kit
缩写：ENPP2
Uniprot No.：Q13822
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 ng/mL-2000 ng/mL
灵敏度：31.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-103 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 86-100 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-101 | |||||
回收率：
| The recovery of human ENPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 82-104 | ||||
| EDTA plasma (n=4) | 97 | 85-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 20000.1450.1420.144 7500.2700.2830.277 3750.4320.4100.421 1250.5710.5920.582 251.0371.0071.022 02.4322.4112.422 | ||||||
货期：3-5 working days
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