首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肾上腺素(EPI)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPI含量。试剂盒检测范围为2 pg/mL-400 pg/mL，适用于神经生物学、内分泌学及药理学等领域的基础研究，例如应激状态下激素分泌机制探索、心血管疾病模型中的激素动态监测，或药物干预对肾上腺功能影响的评价等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：EPI
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：2 pg/mL-400 pg/mL
灵敏度：1 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 80-94 | ||||
| EDTA plasma (n=4) | 98 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 4000.2130.2100.212 1000.4080.3910.400 320.6130.6310.622 81.0621.0031.033 21.5931.5161.555 02.4672.4122.440 | ||||||
货期：3-5 working days
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