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产品描述：猪内皮素1(ET-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EDN1含量。EDN1即内皮素 -1 ，是一种具有强烈而持久血管收缩作用的肽类物质。在生理上，它参与血压调节等过程。研究机制方面，聚焦于其与心血管、呼吸系统等疾病关联，如参与肺动脉高压发展，通过作用于受体调节血管张力等。试剂盒检测范围为3.12 pg/mL-200pg/mL，该产品适用于科研场景中评估猪心血管疾病模型、药物干预效果或组织微环境中ET - 1的动态变化，其宽检测范围和高灵敏度可精准捕捉低丰度样本的浓度差异，为心血管机制研究及药理实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EDN1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
缩写：EDN1
Uniprot No.：P09558
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of pig ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.4012.4492.4252.3541001.6891.6611.6751.604500.9250.9120.9190.848250.4910.4590.4750.40412.50.3020.2860.2940.2236.250.2210.2130.2170.1463.120.1580.1540.1560.08500.0680.0740.071 | ||||||
货期：3-5 working days
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