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产品描述：小鼠凝血酶原片段F1+2(F1+2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F1+2含量。小鼠凝血酶原片段F1+2是凝血酶原激活转化为凝血酶过程中释放的稳定降解产物，其含量反映凝血酶早期激活水平，可用于诊断血栓前状态和血栓性疾病，是血栓性疾病早期诊断的分子标志物。试剂盒检测范围为15.6 pmol/L-1000 pmol/L，常用于评估凝血系统活化状态，在血栓形成机制研究、抗凝药物效果评价及凝血相关疾病动物模型（如心血管疾病模型或遗传性凝血障碍研究）中具有重要科研价值。该产品适用于体外实验，能够灵敏检测多种生物样本中F1+2的动态变化，为凝血与纤溶平衡研究、病理模型建立及药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：F1+2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pmol/L-1000 pmol/L
灵敏度：3.9 pmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 109 | ||||
| Range % | 104-112 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of mouse F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 94-99 | ||||
| EDTA plasma (n=4) | 91 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/LOD1OD2AverageCorrected10002.5522.4002.4762.2885001.7201.7361.7281.5402500.9950.9810.9880.8001250.6160.6540.6350.44762.50.4340.4020.4180.23031.250.3530.3680.3610.17315.60.2990.2870.2930.10500.1890.1870.188 | ||||||
货期：3-5 working days
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