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产品描述：人凝血因子XIIIB链(F13B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的F13B含量。F13B是凝血因子XIII的B亚基，参与凝血级联反应的最后环节，与A亚基共同组成异四聚体。F13B缺乏催化活性，但能稳定A亚基并调节凝血酶激活转谷氨酰胺酶，从而稳定纤维蛋白凝块。F13B基因突变可导致遗传性FXIII缺陷症，表现为终身出血倾向、伤口愈合不良等。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒适用于生命科学领域研究，可用于探究F13B在血栓形成、遗传性凝血缺陷或组织修复等生理病理过程中的动态变化，为心血管疾病模型构建、药物干预效果评估及分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F13B ELISA Kit; Coagulation factor XIII B chain ELISA Kit; Fibrin-stabilizing factor B subunit ELISA Kit; Protein-glutamine gamma-glutamyltransferase B chain ELISA Kit; Transglutaminase B chain ELISA Kit
缩写：F13B
Uniprot No.：P05160
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human F13B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:200 | Average % | 93 | |||||
| Range % | 90-96 | ||||||
| 1:400 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| 1:800 | Average % | 99 | |||||
| Range % | 95-103 | ||||||
回收率：
| The recovery of human F13B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 107 | 103-110 | |||||
| EDTA plasma (n=4) | 89 | 86-91 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.7342.6542.6942.617102.1222.2052.1642.08751.4971.4871.4921.4152.50.8750.8980.8870.8101.250.5330.5680.5510.4740.6250.3310.3520.3420.2650.3120.2060.1980.2020.12500.0790.0750.077 | |||||||
货期：3-5 working days
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