首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织因子(TF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TF含量。TF即组织因子，是一种跨膜糖蛋白。它是凝血系统的重要启动因子，在生理止血中起关键作用。研究发现其异常表达与炎症、肿瘤转移等密切相关，通过激活凝血途径及信号传导等机制参与疾病进展，是潜在的治疗靶点。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于心血管疾病、肿瘤微环境、炎症反应等基础研究领域，帮助科研人员探索TF在疾病发生发展中的作用机制，或作为生物标志物用于实验性治疗方案的效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD142 ELISA Kit; CD142 antigen ELISA Kit; Coagulation factor III (thromboplastin tissue factor) ELISA Kit; Coagulation factor III ELISA Kit; F3 ELISA Kit; FLJ17960 ELISA Kit; TF ELISA Kit; TF_HUMAN ELISA Kit; TFA ELISA Kit; Thromboplastin ELISA Kit; Tissue factor ELISA Kit
缩写：TF
Uniprot No.：P13726
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 98-104 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-103 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.8752.8992.8872.7291002.1532.1672.1602.002501.2751.2431.2591.101250.6580.6120.6350.47712.50.4280.4570.4430.2856.250.3250.3280.3270.1693.120.2410.2610.2510.09300.1590.1570.158 | ||||||
货期：3-5 working days
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