首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血纤蛋白原(Fbg)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Fbg含量。Fbg即纤维蛋白原，在凝血过程中起关键作用。其与血栓形成紧密相关，过高水平易致血栓性疾病。研究机制主要围绕其在凝血级联反应中的作用、与血小板相互作用等方面，探索调节其功能可开发抗血栓等相关治疗策略。试剂盒检测范围为0.156 μg/mL-10 μg/mL，适用于小鼠凝血机制研究、血栓性疾病模型评估、肝脏功能分析或创伤修复相关实验，可为心血管疾病、出血性疾病及炎症反应等科研领域提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Fbg
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.039 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 87-103 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 84-105 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 83-106 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 88-104 | |||||
回收率：
| The recovery of mouse Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-108 | ||||
| EDTA plasma (n=4) | 96 | 88-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected102.6722.7112.6922.55951.9582.0131.9861.8532.51.2751.2821.2791.1461.250.7290.7640.7470.6140.6250.4370.4340.4360.3030.3120.3650.3490.3570.2240.1560.2590.2470.2530.12000.1300.1350.133 | ||||||
货期：3-5 working days
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