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产品描述:牛纤维蛋白原(Fb)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的Fb含量。试剂盒检测范围为3.125 μg/mL-200 μg/mL,本产品适用于动物医学研究、畜牧业健康监测、营养代谢评估等科研领域,特别针对凝血功能异常模型或炎症相关研究中的纤维蛋白原动态变化分析,为科研人员提供稳定、可重复的检测方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:N/A
缩写:Fb
种属:Bos taurus (Bovine)
样本类型:serum, plasma
检测范围:3.125 μg/mL-200 μg/mL
灵敏度:1.56 μg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine Fb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:20 | Average % | 90 | ||||
Range % | 86-97 | |||||
1:40 | Average % | 96 | ||||
Range % | 92-100 | |||||
1:80 | Average % | 85 | ||||
Range % | 81-86 | |||||
1:160 | Average % | 89 | ||||
Range % | 83-98 |
回收率:
The recovery of bovine Fb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 87-95 | ||||
EDTA plasma (n=4) | 96 | 92-100 | ||||
货期:3-5 working days
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