首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠腓骨蛋白-1(FBLN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FBLN1含量。FBLN1（fibulin-1）是一种分泌性糖蛋白，主要存在于细胞外基质中，参与细胞粘附、迁移和信号传导。其研究机制涉及与层粘连蛋白和巢蛋白的结合，以及通过钙结合介导的血小板粘附。FBLN1的表达与多种疾病如动脉粥样硬化、癌症等密切相关。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于研究FBLN1在纤维化疾病模型中的动态变化、肿瘤微环境基质重塑机制、胚胎发育相关信号通路调控等科研领域，支持体外实验中对不同样本类型的FBLN1表达水平进行精确量化分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fbln1Fibulin-1 ELISA kit; FIBL-1 ELISA kit; Basement-membrane protein 90 ELISA kit; BM-90 ELISA kit
缩写：FBLN1
Uniprot No.：Q08879
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 91-101 | |||||
| 1:4000 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of mouse FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-97 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.4192.3192.3692.2031001.7011.6011.6511.485501.0221.0021.0120.846250.6640.6540.6590.49312.50.4480.4250.4370.2716.250.3750.3650.3700.2043.120.2870.2810.2840.11800.1680.1640.166 | ||||||
货期：3-5 working days
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