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产品描述：人原纤维蛋白-3(FBN3)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FBN3含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可应用于心血管发育、结缔组织相关疾病模型或遗传学研究中FBN3蛋白表达的动态监测，为探索其生物学功能及调控机制提供工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FBN3 ELISA Kit; KIAA1776Fibrillin-3 [Cleaved into: Fibrillin-3 C-terminal peptide] ELISA Kit
缩写：FBN3
Uniprot No.：Q75N90
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FBN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-96 | |||||
回收率：
| The recovery of human FBN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 81-93 | ||||
| EDTA plasma (n=4) | 98 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4092.4562.4332.325101.5351.5851.5601.45250.8120.8010.8070.6992.50.5060.5450.5260.4181.250.2720.2650.2690.1610.6250.1660.1550.1610.0530.3120.1500.1580.1540.04600.1040.1110.108 | ||||||
货期：3-5 working days
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