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Human Fibrillin-1 (FBN1)ELISA Kit

  • 中文名： 人原纤维蛋白-1(FBN1)ELISA Kit
货号: IPD-E13141h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人原纤维蛋白-1(FBN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的FBN1含量。FBN1基因位于人类第15号染色体，编码原纤维蛋白-1，参与构成弹性纤维，为结缔组织提供机械支撑。其突变与多种遗传性疾病相关，如马凡综合征（MFS）和孤立性晶状体异位（IEL），研究其功能机制有助于疾病诊断和治疗。试剂盒检测范围为0.235 ng/ml-15 ng/ml.，该产品适用于心血管疾病模型、结缔组织代谢研究及遗传性疾病机制探索等科研领域，能够为体外实验中FBN1表达水平监测、病理生理过程分析及药物干预效果评估提供可靠工具，所有应用仅限科学研究目的。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    350 kDa glycoprotein component extracellular microfibril ELISA Kit; ACMICD ELISA Kit; FBN 1 ELISA Kit; FBN ELISA Kit; FBN1 ELISA Kit; FBN1_HUMAN ELISA Kit; Fibrillin 15 ELISA Kit; Fibrillin-1 ELISA Kit; Fibrillin1 ELISA Kit; Fibrillin15 ELISA Kit; GPHYSD2 ELISA Kit; Marfan syndrome ELISA Kit; MASS ELISA Kit; MFS 1 ELISA Kit; MFS1 ELISA Kit; OCTD ELISA Kit; SGS ELISA Kit; SSKS ELISA Kit; Weill Marchesani syndrome ELISA Kit; WMS ELISA Kit; WMS2 ELISA Kit
  • 缩写：
    FBN1
  • Uniprot No.：
    P35555
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.235 ng/ml-15 ng/ml.
  • 灵敏度：
    0.058 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FBN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    104


    Range %

    96-112


    1:2

    Average %

    95


    Range %

    91-105


    1:4

    Average %

    94


    Range %

    92-110


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human FBN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    99

    90-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    15

    2.748

    2.746

    2.747

    2.652


    7.5

    2.235

    2.256

    2.246

    2.151


    3.75

    1.542

    1.643

    1.593

    1.498


    1.88

    0.906

    0.954

    0.930

    0.835


    0.94

    0.446

    0.467

    0.457

    0.362


    0.47

    0.243

    0.256

    0.250

    0.155


    0.235

    0.152

    0.163

    0.158

    0.063


    0

    0.092

    0.097

    0.095




  • 货期：
    3-5 working days

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