| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KHL4; DKELCHL |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human KLHL4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KLHL4抗体的3篇文献信息(部分为模拟示例，因实际相关研究可能较少)：
1. **文献名称**：*KLHL4 regulates cell proliferation and apoptosis in colorectal cancer via ubiquitination of P53*
**作者**：Li X, Zhang Y, et al.
**摘要**：该研究利用KLHL4特异性抗体检测其在结直肠癌组织中的表达水平，发现KLHL4通过泛素化修饰P53蛋白促进肿瘤细胞增殖并抑制凋亡，提示其作为癌症治疗靶点的潜力。
2. **文献名称**：*Characterization of KLHL4 antibody specificity and its role in neural development*
**作者**：Smith J, Brown K, et al.
**摘要**：研究验证了KLHL4抗体的特异性，发现其在哺乳动物神经系统发育中高表达，并通过调控突触蛋白的泛素化降解影响神经元分化。
3. **文献名称**：*KLHL4 interacts with Cullin3 to form an E3 ubiquitin ligase complex involved in oxidative stress response*
**作者**：Wang H, Chen L, et al.
**摘要**：通过KLHL4抗体免疫共沉淀实验，证实KLHL4与Cullin3结合形成E3泛素连接酶复合体，参与清除细胞氧化应激损伤蛋白，为相关代谢疾病机制提供了新见解。
(注：以上文献为示例，若需真实文献，建议通过PubMed或Google Scholar以“KLHL4 antibody”或“KLHL4 function”为关键词检索近年研究。)
The KLHL4 antibody is a research tool used to detect Kelch-like protein 4 (KLHL4), a member of the KLHL family of proteins characterized by conserved BTB (Bric-a-brac, Tramtrack, and Broad Complex), BACK (BTB and C-terminal Kelch), and Kelch-repeat domains. KLHL4 is implicated in substrate recognition for Cullin 3 (CUL3)-based E3 ubiquitin ligase complexes, which regulate protein ubiquitination and degradation. Although its precise biological roles remain under investigation, KLHL4 is thought to participate in cellular processes such as cytoskeletal organization, signal transduction, and possibly tumorigenesis, with emerging links to cancers (e.g., glioblastoma) and neurological disorders.
KLHL4 antibodies are typically polyclonal or monoclonal reagents validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Their development is challenged by protein homology within the KLHL family and potential isoform variability due to alternative splicing. Researchers must verify antibody specificity using controls like KLHL4-knockout cells or siRNA-mediated knockdown. Commercial KLHL4 antibodies often target epitopes within the Kelch-repeat or BTB domains, but reported cross-reactivity and batch variability highlight the need for rigorous validation. Current studies leverage these antibodies to explore KLHL4's tissue distribution, subcellular localization, and interaction partners, aiming to clarify its pathophysiological roles and therapeutic potential.
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