首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人腓骨蛋白-5(FBLN5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FBLN5含量。FBLN5是纤连蛋白家族成员，在多种组织细胞外基质中表达。它参与维持组织弹性结构。研究发现其在肿瘤、心血管疾病中作用关键，机制涉及调控细胞黏附、迁移、增殖等，有望成疾病治疗新靶点，为后续治疗提供新思路。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于细胞实验、疾病模型或组织工程研究中FBLN5表达水平的动态监测，例如探究其在组织纤维化、血管弹性调节或肿瘤侵袭中的作用机制；在皮肤老化、心血管疾病及肿瘤微环境调控等研究中具有重要科研价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADCL2 ELISA Kit; ARCL1A ELISA Kit; ARMD3 ELISA Kit; Dance ELISA Kit; Developmental arteries and neural crest EGF like protein ELISA Kit; Developmental arteries and neural crest EGF-like protein ELISA Kit; EVEC ELISA Kit; Fbln5 ELISA Kit; FBLN5_HUMAN ELISA Kit; FIBL 5 ELISA Kit; FIBL-5 ELISA Kit; Fibulin-5 ELISA Kit; FLJ90059 ELISA Kit; UP50 ELISA Kit; Urine p50 protein ELISA Kit
缩写：FBLN5
Uniprot No.：Q9UBX5
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of human FBLN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.7882.7592.7742.63920002.5882.3792.4842.34910002.0802.0052.0431.9085001.5701.5021.5361.4012500.7330.7530.7430.6081250.3420.3530.3480.21362.50.2150.2250.2200.08500.1300.1390.135 | ||||||
货期：3-5 working days
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