首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述:人高亲和免疫球蛋白γFc受体I(FCGR1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FCGR1A含量。FCGR1A是重要靶点。它在免疫调节等生理过程中有关键作用。研究发现其与多种免疫相关疾病的发生发展机制存在联系,如在炎症反应调控上有特殊机制。对它的深入研究有助于解析疾病发病机制,为相关疾病治疗提供新方向。试剂盒检测范围为0.78 ng/mL-50 ng/mL,本试剂盒适用于科研领域中对FCGR1A蛋白表达动态的精准分析,例如通过血清或血浆样本评估免疫系统活性,或利用组织匀浆研究特定病理状态下受体表达的变化规律。支持研究者探索FCGR1A在免疫应答机制、药物干预效果或疾病生物标志物筛选等方向的应用。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:FCGR1A; FCG1; FCGR1; IGFR1; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD antigen CD64
缩写:FCGR1A
Uniprot No.:P12314
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.78 ng/mL-50 ng/mL
灵敏度:0.2 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human FCGR1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 95 | |||||
Range % | 90-100 | ||||||
1:2 | Average % | 88 | |||||
Range % | 84-92 | ||||||
1:4 | Average % | 97 | |||||
Range % | 92-101 | ||||||
1:8 | Average % | 102 | |||||
Range % | 98-107 |
回收率:
The recovery of human FCGR1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 84 | 80-88 | |||||
EDTA plasma (n=4) | 96 | 91-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected502.6712.5152.5932.443252.0041.9731.9891.83912.51.4391.4841.4621.3126.250.9270.9420.9350.7853.120.5130.5270.5200.3701.560.3580.3690.3640.2140.780.2620.2510.2570.10700.1510.1480.150 |
货期:3-5 working days
×