首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人低亲和力免疫球蛋白γFc区受体II-b(FCGR2B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FCGR2B含量。FCGR2B是一个重要靶点。它在免疫调节等方面发挥关键作用。研究发现其能够影响免疫细胞的活性与功能，参与多种免疫相关疾病的发生发展，深入探究其机制，有望为免疫疾病的治疗提供新的思路和潜在药物靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研场景中评估不同样本类型的FCGR2B蛋白表达差异，例如自身免疫疾病模型研究、感染后免疫应答分析或肿瘤微环境免疫调节机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FCGR2B; CD32; FCG2; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD antigen CD32
缩写：FCGR2B
Uniprot No.：P31994
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FCGR2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 93-101 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 96-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human FCGR2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 104 | 99-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.7352.7112.7232.580201.7381.7861.7621.619101.1631.1881.1761.03350.7150.7230.7190.5762.50.4950.4880.4920.3491.250.3360.3280.3320.1890.6250.2420.2370.2400.09700.1440.1420.143 | ||||||
货期：3-5 working days
×
