首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人低亲和力免疫球蛋白γFc区受体III-A(FCGR3A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FCGR3A含量。FCGR3A是重要的免疫相关靶点，在免疫细胞表面表达。其背景与免疫调节密切相关，可结合免疫复合物。研究机制方面，聚焦于其在抗体依赖细胞介导的细胞毒性作用中的角色，了解其信号传导机制有望为免疫治疗药物研发等提供新方向。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于科研场景中探究FCGR3A在自身免疫性疾病、感染免疫调控或肿瘤免疫微环境中的作用机制，也可用于评估生物治疗药物对受体表达水平的影响。为免疫学、肿瘤学及药物开发等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD antigen CD16a
缩写：FCGR3A
Uniprot No.：P08637
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FCGR3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-101 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of human FCGR3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 80-98 | ||||
| EDTA plasma (n=4) | 92 | 85-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.4562.5642.5102.39015002.0751.9652.0201.9007501.2361.3161.2761.1563750.6120.6260.6190.499187.50.3240.3320.3280.208940.2510.2460.2490.129470.1870.1960.1920.07200.1210.1190.120 | ||||||
货期：3-5 working days
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