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产品描述:人低亲和力免疫球蛋白γFc区受体III-B(FCGR3B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FCGR3B含量。FCGR3B是重要的免疫相关靶点。它在免疫细胞表面表达,参与抗体依赖的细胞介导的细胞毒性作用等免疫过程。其异常表达或功能失调与自身免疫性疾病、感染性疾病等发生发展相关,研究围绕调节其活性来开发潜在治疗手段。试剂盒检测范围为1.56 ng/mL-100 ng/mL,适用于基础科研中探索FCGR3B在系统性红斑狼疮、类风湿性关节炎等疾病中的调控机制,或用于评估药物干预对中性粒细胞功能的影响,为免疫相关疾病的机制研究与治疗策略开发提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:CD16 ELISA Kit; CD16b ELISA Kit; CD16b antigen ELISA Kit; Fc fragment of IgG low affinity IIIb receptor ELISA Kit; Fc gamma receptor IIIb ELISA Kit; Fc gamma RIII ELISA Kit; Fc gamma RIII beta ELISA Kit; Fc gamma RIIIb ELISA Kit; Fc-gamma RIII ELISA Kit; Fc-gamma RIII-beta ELISA Kit; Fc-gamma RIIIb ELISA Kit; FCG3 ELISA Kit; FCG3B_HUMAN ELISA Kit; FCGR3 ELISA Kit; FCGR3B ELISA Kit; FcR 10 ELISA Kit; FcR-10 ELISA Kit; FcR10 ELISA Kit; FcRIII ELISA Kit; FcRIIIb ELISA Kit; IGFR3 ELISA Kit; IgG Fc receptor III 1 ELISA Kit; IgG Fc receptor III-1 ELISA Kit; Low affinity immunoglobulin gamma Fc region receptor III B ELISA Kit; Low affinity immunoglobulin gamma Fc region receptor III-B ELISA Kit
缩写:FCGR3B
Uniprot No.:O75015
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:1.56 ng/mL-100 ng/mL
灵敏度:0.39 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human FCGR3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:20 | Average % | 92 | ||||
Range % | 86-100 | |||||
1:40 | Average % | 97 | ||||
Range % | 90-104 | |||||
1:80 | Average % | 95 | ||||
Range % | 87-99 | |||||
1:160 | Average % | 93 | ||||
Range % | 90-99 |
回收率:
The recovery of human FCGR3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 87-98 | ||||
EDTA plasma (n=4) | 93 | 90-96 | ||||
货期:3-5 working days
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