首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人IgG受体FcRn大亚基p51(FCGRT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FCGRT含量。FCGRT（FcγRn）是一种细胞表面受体，通过与免疫球蛋白G（IgG）的Fc区结合，选择性摄取牛奶中的单体IgG，传递被动体液免疫。FCGRT在肠上皮上结合IgG，形成FcRn-IgG复合物，通过转胞吞作用将IgG释放到血液或组织中，对被动体液免疫转移起关键作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于生命科学研究中FcRn相关功能机制探索、抗体药效评估或免疫代谢调控实验。可为免疫学、生物制药等领域研究人员提供FcRn系统的分子水平检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha chain ELISA Kit; Fc fragment of IgG; receptor transporter; alpha ELISA Kit; FCGRN_HUMAN ELISA Kit; FCGRT ELISA Kit; FcRn alpha chain ELISA Kit; FcRn ELISA Kit; FCRN; alpha chain ELISA Kit; IgG Fc fragment receptor transporter alpha chain ELISA Kit; IgG Gc receptor ELISA Kit; IgG receptor FcRn large subunit p51 ELISA Kit; IgG receptor FcRn large subunit p51 precursor ELISA Kit; Immunoglobulin receptor; intestinal; heavy chain ELISA Kit; Neonatal Fc receptor ELISA Kit
缩写：FCGRT
Uniprot No.：P55899
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FCGRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human FCGRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 84-98 | ||||
| EDTA plasma (n=4) | 88 | 85-93 | ||||
货期：3-5 working days
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