关键信息
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基因名
SRPK2
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简介
SRPK2 is a serine/arginine-rich protein-specific kinase that phosphorylates serine residues within the arginine/serine-rich domain (RS domain) and plays a critical role in regulating the splicing process effect. SRPK2 promotes neuronal apoptosis by upregulating cyclin-D1 (CCND1) expression and inhibiting the inhibitory effect of p53/TP53. SRPK2 Protein, Human (sf9, GST) is the recombinant human-derived SRPK2 protein, expressed by sf9 insect cells , with N-GST labeled tag.
- 应用
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别名
SRPK2; SRSF protein kinase 2; SFRS protein kinase 2; Serine/arginine-rich protein-specific kinase 2; SR-protein-specific kinase 2
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种属
Human
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表达系统
Baculovirus
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标签
N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P78362
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表达区间
S2-N688
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SRPK2 (Serine/Arginine-rich protein kinase 2) is a crucial member of the SR protein kinase family, which plays a significant role in the phosphorylation of serine/arginine-rich splicing factors, thereby modulating pre-mRNA splicing and influencing gene expression. The importance of SRPK2 has been highlighted in various biological processes, including cell proliferation, differentiation, and response to stress. Dysregulation of SRPK2 has been implicated in several diseases, particularly cancer, where altered splicing patterns can lead to tumor progression and metastasis. Moreover, understanding the precise mechanisms by which SRPK2 regulates splicing has significant implications for therapeutic strategies targeting splicing-related pathologies. In recent years, there has been growing interest in the recombinant production of SRPK2 to facilitate biochemical studies aimed at uncovering its functional roles and regulatory mechanisms. The generation of recombinant SRPK2 proteins allows for detailed analyses of its kinase activity, substrate specificity, and interaction with splicing factors. Such studies are essential for elucidating the complex regulatory networks involving SRPK2, providing insights that could lead to novel diagnostic or therapeutic approaches in diseases linked to splicing abnormalities. As research continues to expand, the investigation of SRPK2 through recombinant protein technology remains a promising avenue for uncovering its potential as a target in the development of splicing-focused therapies.
