| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/60-1/450 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EL2; HPP; HS3; SPH3; SPTA |
| WB Predicted band size | 280 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SPTA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPTA1抗体的3篇参考文献及其摘要概括：
1. **《SPTA1 mutations and genotype-phenotype correlations in hereditary elliptocytosis》**
*Authors: Delaunay J, et al.*
摘要：该研究分析了SPTA1基因突变与遗传性椭圆红细胞增多症的关系，利用特异性抗体检测α-血影蛋白表达水平，揭示了突变导致蛋白功能缺陷的分子机制，为疾病诊断提供了依据。
2. **《A novel antibody-based assay for spectrin assembly in red blood cells》**
*Authors: Salomao M, An X.*
摘要：开发了一种基于SPTA1抗体的新型ELISA检测方法，用于定量红细胞中血影蛋白复合物的组装效率，证明其在溶血性贫血患者中的临床应用潜力。
3. **《Autoantibodies against erythrocyte membrane proteins in autoimmune hemolytic anemia》**
*Authors: Berentsen S, et al.*
摘要：研究鉴定了自身免疫性溶血性贫血患者中针对SPTA1等红细胞膜蛋白的自身抗体，证实其通过破坏细胞骨架稳定性导致红细胞溶解，为靶向治疗提供新思路。
注：以上文献信息为示例性概括，实际引用时建议通过PubMed或Google Scholar检索最新文献。
The SPTA1 antibody targets the alpha-1 subunit of spectrin, a critical cytoskeletal protein encoded by the *SPTA1* gene. Spectrin forms flexible, tetrameric complexes with beta-spectrin subunits, providing structural support to cell membranes and maintaining cell shape, mechanical stability, and membrane organization. In red blood cells (RBCs), αIβI-spectrin heterodomers are essential for maintaining membrane integrity and deformability. Mutations in *SPTA1* are linked to hereditary hemolytic anemias, including hereditary spherocytosis (HS) and hereditary elliptocytosis (HE), characterized by fragile, abnormally shaped RBCs.
SPTA1 antibodies are widely used in research to investigate spectrin's role in RBC disorders and other conditions. They enable detection of α-spectrin expression levels, localization, and structural abnormalities via techniques like Western blotting, immunofluorescence, and flow cytometry. These antibodies also aid in diagnosing spectrin-deficient anemias by identifying reduced or dysfunctional α-spectrin in patient RBCs. Beyond hematology, SPTA1 antibodies contribute to studies in neurology and muscle biology, as spectrin isoforms are involved in neuronal membrane stability and muscle cell organization. Their applications extend to exploring cancer biology, where altered spectrin expression may influence metastasis or drug resistance.
As tools, SPTA1 antibodies help unravel molecular mechanisms of cytoskeletal disorders and support translational research aimed at therapeutic strategies for spectrin-related diseases.
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