首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人成纤维细胞生长因子10(FGF10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF10含量。FGF10即成纤维细胞生长因子 10，属于FGF家族成员。它在胚胎发育、组织修复等生理过程中发挥重要作用。研究机制方面，其主要通过与受体结合激活下游信号通路，影响细胞增殖、分化、迁移等，在多种疾病如癌症、肺疾病等研究中受关注。试剂盒检测范围为3.12 pg/mL-200 pg/mL，支持科研人员探究FGF10在肺纤维化、慢性炎症性疾病模型中的表达动态，或用于干细胞分化、再生医学等基础研究领域，为解析FGF10信号通路及其在组织稳态中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BB213776 ELISA Kit; fd11d03 ELISA Kit; FGF 10 ELISA Kit; FGF-10 ELISA Kit; FGF10 ELISA Kit; FGF10_HUMAN ELISA Kit; Fibroblast growth factor 10 ELISA Kit; Keratinocyte growth factor 2 ELISA Kit; KGF 2 ELISA Kit; Produced by fibroblasts of urinary bladder lamina propria ELISA Kit; wu:fd11d03 ELISA Kit; zgc:109774 ELISA Kit
缩写：FGF10
Uniprot No.：O15520
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:10 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:20 | Average % | 100 | ||||
| Range % | 96-105 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of human FGF10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 97 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.8722.9392.9062.7151002.3762.4132.3952.204501.6621.5631.6131.422251.0871.0481.0680.87712.50.6040.6510.6280.4376.250.4740.4390.4570.2663.120.3050.2890.2970.10600.1920.1900.191 | ||||||
货期：3-5 working days
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