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Human Fibroblast growth factor 17(FGF17) ELISA kit

  • 中文名: 人成纤维细胞生长因子17(FGF17)酶联免疫试剂盒
货号: IPD-EL008622HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人成纤维细胞生长因子17(FGF17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、组织培养上清液.样本中的FGF17含量。FGF17是一种成纤维细胞生长因子,在胚胎发育等过程中发挥重要作用。其研究机制聚焦于它与受体结合后激活下游信号通路,调控细胞增殖、分化等。在肿瘤等疾病中可能参与发生发展,为相关治疗提供潜在靶点。试剂盒检测范围为3.12 pg/ml-200 pg/ml.,研究者可将其应用于发育生物学、疾病机制研究或药物干预实验中FGF17表达水平的动态监测,为探索其生理功能及病理意义提供可靠的工具支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    FGF 13 ELISA Kit; FGF 17 ELISA Kit; FGF-17 ELISA Kit; FGF13 ELISA Kit; Fgf17 ELISA Kit; FGF17_HUMAN ELISA Kit; Fibroblast growth factor 17 ELISA Kit; HH20 ELISA Kit
  • 缩写:
    FGF17
  • Uniprot No.:
    O60258
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates, cell culture supernates.
  • 检测范围:
    3.12 pg/ml-200 pg/ml.
  • 灵敏度:
    0.78 pg/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human FGF17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    85-96


    1:2

    Average %

    95


    Range %

    88-101


    1:4

    Average %

    100


    Range %

    93-105


    1:8

    Average %

    93


    Range %

    86-101


  • 回收率:

    The recovery of human FGF17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    86-100


    EDTA plasma (n=4)

    97

    92-103


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    200

    2.264

    2.234

    2.249

    2.078


    100

    1.582

    1.667

    1.625

    1.454


    50

    0.986

    1.028

    1.007

    0.836


    25

    0.660

    0.629

    0.645

    0.474


    12.5

    0.453

    0.431

    0.442

    0.271


    6.25

    0.338

    0.343

    0.341

    0.170


    3.12

    0.251

    0.244

    0.247

    0.077


    0

    0.166

    0.175

    0.171




  • 货期:
    3-5 working days

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