首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人成纤维细胞生长因子13(FGF-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的FGF13含量。FGF13 即成纤维细胞生长因子 13，在人体发育与疾病发生机制中扮演关键角色。研究发现，它通过参与细胞增殖、分化和迁移等过程影响生理病理状态，和神经系统发育、肿瘤发展等密切相关，是极具潜力的药物研发靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于神经生物学、细胞信号传导机制研究等科研场景，支持体外培养细胞模型中FGF - 13分泌水平的动态监测，或生物体液样本的定量分析，为探索FGF - 13在神经退行性疾病、发育异常等领域的生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF 13 ELISA Kit; FGF 2 ELISA Kit; FGF-13 ELISA Kit; FGF13 ELISA Kit; FGF13_HUMAN ELISA Kit; FGF2 ELISA Kit; FHF 2 ELISA Kit; FHF-2 ELISA Kit; FHF2 ELISA Kit; Fibroblast growth factor 13 ELISA Kit; Fibroblast growth factor homologous factor 2 ELISA Kit; OTTHUMP00000024143 ELISA Kit; OTTHUMP00000024144 ELISA Kit
缩写：FGF13
Uniprot No.：Q92913
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of human FGF-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 104 | 100-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected8002.1522.2632.2082.0624001.6071.7121.6601.5142001.1791.0961.1380.9921000.7810.7450.7630.617500.4350.4510.4430.297250.3140.3070.3110.16512.50.2280.2340.2310.08500.1430.1490.146 | |||||||
货期：3-5 working days
×
