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产品描述：小鼠成纤维细胞生长因子10(FGF10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FGF10含量。FGF10，也称为成纤维细胞生长因子10，是一种肝素结合生长因子，属于FGF家族。它在多种生物过程中发挥重要作用，包括胚胎发育、细胞生长、形态发生、组织修复和肿瘤生长。FGF10通过与其受体FGFR结合，激活信号通路，从而影响细胞增殖、分化和迁移。研究显示，FGF10在心脏修复、脂肪生成和器官形态发生中具有关键作用。试剂盒检测范围为1.56 pg/mL-100 pg/mL，可广泛应用于细胞因子分泌研究、组织微环境分析、疾病模型建立等科研领域，尤其适用于发育生物学、再生医学及炎症相关机制研究中的FGF10动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgf10Fibroblast growth factor 10 ELISA Kit; FGF-10 ELISA Kit; Keratinocyte growth factor 2 ELISA Kit
缩写：FGF10
Uniprot No.：O35565
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of mouse FGF10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 98-108 | ||||
| EDTA plasma (n=4) | 95 | 90-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.1942.3452.2702.102501.4941.4651.4801.312250.8890.9670.9280.76012.50.5940.5560.5750.4076.250.3840.3830.3840.2163.120.3020.2900.2960.1281.560.2120.2250.2190.05100.1670.1680.168 | ||||||
货期：3-5 working days
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