首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人角化细胞生长因子(KGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF7含量。FGF7（成纤维细胞生长因子7）是一种分泌型糖蛋白，属于FGF家族成员。它由间质来源细胞产生，特异性结合上皮细胞表达的Fgfr2b受体，介导间质-上皮信号。FGF7在胚胎发育、细胞增殖、形态发生和组织修复中起重要作用，并参与肿瘤生长和侵袭。研究显示，FGF7的表达与肿瘤进展和转移密切相关，因此成为潜在的治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，具有高灵敏度和特异性，适用于多种生物样本类型，可为科研人员探究KGF在皮肤再生机制、黏膜修复过程或疾病模型（如肺纤维化、肠道炎症等）中的动态变化提供可靠工具，支持基础医学、药理学及再生医学领域的研究，例如药物干预效果评估或病理机制解析，为相关科研实验提供精准的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF 7 ELISA Kit; FGF-7 ELISA Kit; Fgf7 ELISA Kit; FGF7_HUMAN ELISA Kit; Fibroblast growth factor 7 ELISA Kit; HBGF 7 ELISA Kit; HBGF-7 ELISA Kit; HBGF7 ELISA Kit; Heparin binding growth factor 7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; Keratinocyte growth factor ELISA Kit; KGF ELISA Kit
缩写：FGF7
Uniprot No.：P21781
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 86 | 81-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.4132.5422.4782.362 10001.9411.8481.8951.779 5001.2121.2351.2241.108 2500.7640.7520.7580.642 1250.4410.4560.4490.333 62.50.2970.2840.2910.175 31.250.1870.1810.1840.068 00.1150.1160.116 | ||||||
货期：3-5 working days
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