首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人卵巢滤泡激素(FLCN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FLCN含量。FLCN是重要靶点。它在细胞代谢、增殖等过程有关键作用，其功能异常与多种疾病发生发展相关。研究聚焦于解析FLCN信号通路和调控机制，探究其与疾病关联，为开发针对性治疗策略、药物研发提供依据。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可广泛应用于基础研究领域，例如卵巢生理机制探索、疾病模型构建、药物靶点筛选以及肿瘤微环境分析等。支持科研人员在体外实验中定量评估 FLCN 的表达水平变化，为相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BHD ELISA Kit; BHD skin lesion fibrofolliculoma protein ELISA Kit; Birt Hogg Dube syndrome protein ELISA Kit; Birt-Hogg-Dube syndrome protein ELISA Kit; DKFZp547A118 ELISA Kit; FLCL ELISA Kit; Flcn ELISA Kit; FLCN_HUMAN ELISA Kit; FLJ45004 ELISA Kit; FLJ99377 ELISA Kit; Folliculin ELISA Kit; MGC17998 ELISA Kit; MGC23445 ELISA Kit
缩写：FLCN
Uniprot No.：Q8NFG4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FLCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-105 | |||||
回收率：
| The recovery of human FLCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 96-106 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4342.5332.4842.3385001.6951.5981.6471.5012501.0791.0971.0880.9421250.7070.6860.6970.55162.50.4360.4550.4460.30031.20.3210.3170.3190.17315.60.2550.2480.25200.1470.1450.146 | ||||||
货期：3-5 working days
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