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Mouse Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

  • 中文名： 小鼠血管内皮细胞生长因子D(VEGF-D)ELISA Kit
货号: IPD-E07357m
Price： ￥2500
数量：
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产品详情

  • 产品描述：小鼠血管内皮细胞生长因子D(VEGF-D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Vegfd含量。VEGF-D（血管内皮生长因子D）是一种促进血管和淋巴管生成的生长因子，属于PDGF/VEGF家族。它在血管生成和淋巴管生成中发挥重要作用，与肿瘤生长和转移密切相关。VEGF-D通过激活VEGFR-2和VEGFR-3受体，促进内皮细胞的增殖、迁移和存活，从而促进血管和淋巴管的生成。试剂盒检测范围为0.312 pg/mL-20 pg/mL，该产品可广泛应用于肿瘤生物学、血管生成机制、药物筛选等基础研究领域，为探索VEGF - D在肿瘤微环境调控、血管新生相关疾病模型中的作用提供可靠的检测工具，特别适用于体外培养细胞模型中生长因子分泌水平的动态监测及动物实验样本的批量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Vegfd ELISA Kit; Figf ELISA Kit; Vascular endothelial growth factor D ELISA Kit; VEGF-D ELISA Kit; c-Fos-induced growth factor ELISA Kit; FIGF ELISA Kit

  • 缩写：FIGF

  • Uniprot No.：P97946

  • 种属：Mus musculus (Mouse)

  • 样本类型：serum, plasma, cell culture supernates, tissue homogenates

  • 检测范围：0.312 pg/mL-20 pg/mL

  • 灵敏度：0.078 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Cardiovascular

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:100Average %95
    Range %90-99
    1:200Average %90
    Range %85-96
    1:400Average %96
    Range %82-105
    1:800Average %91
    Range %85-99

  • 回收率：

    The recovery of mouse VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9088-94
    EDTA plasma (n=4)9589-100














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected 202.8572.7052.7812.647 102.3122.2392.2762.142 51.5941.5311.5631.429 2.50.8730.8440.8590.725 1.250.5500.5680.5590.425 0.6250.3410.3440.3430.209 0.3120.2580.2490.2540.120 00.1330.1350.134　 






  • 货期：3-5 working days

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