| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CELF-5; BRUNOL5; BRUNOL-5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CELF5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CELF5抗体的虚构参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**：*Characterization of a Novel CELF5 Antibody for RNA-Binding Studies in Neuronal Cells*
**作者**：Smith A, et al.
**摘要**：本研究开发并验证了一种高特异性CELF5单克隆抗体，用于检测神经元细胞中CELF5蛋白的RNA结合活性。通过免疫沉淀和Western blot分析，证实该抗体在探究CELF5调控突触蛋白mRNA剪接中的应用价值。
2. **文献名称**：*CELF5 Expression Dysregulation in Autism Spectrum Disorder: Insights from Immunohistochemical Analysis*
**作者**：Chen L, et al.
**摘要**：利用CELF5抗体对自闭症患者脑组织样本进行免疫组化分析，发现前额叶皮层中CELF5蛋白表达显著降低，提示其可能与神经发育异常及自闭症病理机制相关。
3. **文献名称**：*CELF5 Antibody Validation in a Mouse Model of ALS*
**作者**：Rodriguez M, et al.
**摘要**：在肌萎缩侧索硬化症(ALS)转基因小鼠模型中，通过CELF5抗体检测到脊髓运动神经元内CELF5蛋白聚集现象，支持CELF5异常调控RNA代谢参与ALS病程的假说。
4. **文献名称**：*Development of a Phospho-Specific CELF5 Antibody for Post-Translational Modification Studies*
**作者**：Tanaka K, et al.
**摘要**：报道了一种针对CELF5磷酸化位点的抗体开发，证实其在细胞应激条件下(如氧化损伤)可特异性识别CELF5的翻译后修饰，为研究CELF5功能调控提供了新工具。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
CELF5 (CUGBP Elav-like family member 5), also known as BRUNOL5. is a RNA-binding protein belonging to the CELF family, which plays critical roles in post-transcriptional gene regulation. This family shares homology with *C. elegans* protein UNC-75 and is characterized by three RNA recognition motifs (RRMs) that mediate interactions with RNA targets. CELF5 is particularly enriched in neuronal tissues and has been implicated in regulating alternative splicing, mRNA stability, and translation. Studies suggest its involvement in neuronal development, synaptic plasticity, and RNA metabolism, with dysregulation linked to neurodevelopmental disorders, including autism spectrum disorders and intellectual disabilities.
Antibodies targeting CELF5 are essential tools for investigating its expression patterns, subcellular localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation. Research utilizing CELF5 antibodies has revealed its nuclear-cytoplasmic shuttling dynamics and association with RNA granules, highlighting its role in stress responses and neuronal RNA homeostasis. Additionally, CELF5 has been studied in the context of cancer, where aberrant expression may influence tumor progression via RNA processing pathways. Despite its established functions, the precise mechanisms of CELF5 in disease pathogenesis remain under active exploration, necessitating reliable antibody reagents for further mechanistic and translational studies.
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