| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ORFX; RING3L |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human BRD3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BRD3抗体的3篇示例文献(注：文献为假设性示例，实际文献需通过数据库检索确认)：
1. **文献名称**：*Structural basis for BRD3 antibody specificity in chromatin recognition*
**作者**：Smith A, et al.
**摘要**：本研究解析了BRD3蛋白与特定抗体的复合物晶体结构，揭示了抗体通过靶向BRD3的乙酰化赖氨酸结合口袋实现高特异性识别，为表观遗传学研究工具开发提供依据。
2. **文献名称**：*BRD3 antibody-based profiling reveals its role in acute myeloid leukemia progression*
**作者**：Chen L, et al.
**摘要**：通过开发高灵敏度BRD3抗体，作者发现BRD3在白血病细胞中异常富集，并通过调控MYC等致癌基因表达促进肿瘤增殖，提示其作为治疗靶点的潜力。
3. **文献名称**：*A comparative study of commercial BRD3 antibodies for ChIP-seq applications*
**作者**：Johnson R, et al.
**摘要**：评估了多种市售BRD3抗体在染色质免疫沉淀(ChIP-seq)中的性能，发现抗体A在特异性和信号强度上表现最优，为相关实验提供选择指南。
4. **文献名称**：*BRD3 antibody-drug conjugate suppresses triple-negative breast cancer growth*
**作者**：Wang Y, et al.
**摘要**：研究利用BRD3特异性抗体偶联化疗药物，在三阴性乳腺癌模型中显著抑制肿瘤生长，证明靶向BRD3的抗体偶联药物策略的可行性。
建议通过PubMed或Google Scholar以“BRD3 antibody”、“BRD3 chromatin”等关键词检索真实文献。
BRD3 (Bromodomain-containing protein 3) is a member of the BET (bromodomain and extraterminal) protein family, which plays a key role in epigenetic regulation by recognizing acetylated lysine residues on histones and other proteins. As an epigenetic "reader," BRD3 binds to acetylated chromatin via its two bromodomains, facilitating transcriptional activation or repression of genes involved in cell proliferation, inflammation, and oncogenesis. Its dysregulation has been linked to cancers, inflammatory diseases, and viral infections.
BRD3 antibodies are essential tools for studying its function, localization, and interactions in both normal and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP) to detect BRD3 expression, map chromatin-binding sites, or identify protein complexes. High-quality BRD3 antibodies exhibit specificity for unique epitopes, distinguishing BRD3 from closely related BET family members like BRD2 and BRD4. Validation across applications and species (e.g., human, mouse) is critical to ensure reliability.
Research on BRD3 has gained momentum due to the therapeutic potential of BET inhibitors in cancer and inflammatory disorders. BRD3-specific antibodies help dissect its distinct biological roles, assess inhibitor efficacy, and explore compensatory mechanisms in drug resistance. However, cross-reactivity and batch variability remain challenges, emphasizing the need for rigorous antibody validation in experimental systems.
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