首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人丝聚合蛋白(FLG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FLG含量。FLG即丝聚蛋白，是皮肤角质形成细胞中重要的结构蛋白。它在维持皮肤屏障功能方面起关键作用。研究发现其基因缺陷会导致皮肤屏障受损，增加外界刺激物进入，与特应性皮炎、银屑病等多种皮肤疾病的发病机制密切相关。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于皮肤生物学研究、疾病机制探索或药物干预实验中表皮屏障相关指标的定量分析；为研究皮肤生理病理过程中FLG的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATOD2 ELISA Kit; Epidermal filaggrin ELISA Kit; FILA_HUMAN ELISA Kit; Filaggrin ELISA Kit; Filaggrin precursor ELISA Kit; Fillagrin ELISA Kit; FLG ELISA Kit; Profilaggrin ELISA Kit
缩写：FLG
Uniprot No.：P20930
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-105 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 100-110 | |||||
回收率：
| The recovery of human FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-98 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 15002.8532.6872.7702.634 7502.4652.3432.4042.268 3751.7861.7121.7491.613 187.50.8950.8650.8800.744 940.4910.4780.4850.349 470.2990.2880.2940.158 23.50.2120.2080.2100.074 00.1370.1350.136 | ||||||
货期：3-5 working days
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