首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒

Mouse Vascular endothelial cell growth factor receptor 3 (VEGFR-3/Flt-4) ELISA kit

  • 中文名： 小鼠血管内皮细胞生长因子受体-3(VEGFR-3/Flt-4)ELISA Kit
货号: IPD-E04766m
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    小鼠血管内皮细胞生长因子受体-3(VEGFR-3/Flt-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FLT4含量。FLT4即Fms样酪氨酸激酶4，是血管内皮生长因子受体家族成员。它在淋巴管生成中发挥关键作用，与肿瘤转移、淋巴水肿等疾病密切相关。研究围绕其信号通路，探索抑制或激活机制，有望为相关疾病治疗提供新思路和靶点。试剂盒检测范围为78 pg/ml-5000 pg/ml.，灵敏度为19.5 pg/ml.。适用于肿瘤微环境研究、淋巴管发育机制探索、转基因动物模型验证及抗血管生成药物开发等科研领域，为探究VEGFR - 3在肿瘤转移、炎症反应或遗传性淋巴水肿等病理过程中的表达动态提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Flt4 ELISA Kit; Flt-4 ELISA Kit; Vegfr3 ELISA Kit; Vascular endothelial growth factor receptor 3 ELISA Kit; VEGFR-3 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fms-like tyrosine kinase 4 ELISA Kit; FLT-4 ELISA Kit; Tyrosine-protein kinase receptor FLT4 ELISA Kit
  • 缩写：
    FLT4
  • Uniprot No.：
    P35917
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/ml-5000 pg/ml.
  • 灵敏度：
    19.5 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-3/Flt-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    88-99


    1:2

    Average %

    90


    Range %

    85-97


    1:4

    Average %

    96


    Range %

    90-102


    1:8

    Average %

    98


    Range %

    95-106


  • 回收率：

    The recovery of mouse VEGFR-3/Flt-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    92-103


    EDTA plasma (n=4)

    99

    94-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    3.017

    2.834

    2.925

    2.804


    2500

    2.300

    2.207

    2.254

    2.132


    1250

    1.508

    1.408

    1.458

    1.336


    625

    0.843

    0.829

    0.836

    0.714


    312

    0.524

    0.521

    0.523

    0.401


    156

    0.351

    0.353

    0.352

    0.230


    78

    0.227

    0.214

    0.221

    0.099


    0

    0.119

    0.125

    0.122




  • 货期：
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×