首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管内皮细胞生长因子受体1(VEGFR-1/Flt1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFR-1/Flt1含量。VEGFR-1/Flt1作为靶点，在血管生成等生理过程中起重要作用。其背景在于它是血管内皮生长因子受体家族成员。研究机制方面，主要聚焦于其信号传导通路，通过与配体结合传递信号，影响细胞的增殖、迁移等，对肿瘤血管生成等疾病研究有重要意义。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用各类血管相关疾病模型的机制研究，如肿瘤血管生成实验、心血管病理分析或药物干预后受体表达水平评估，特别适用于需要高灵敏度检测血管内皮细胞功能标志物的基础研究领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Flt1 ELISA Kit; Flt-1 ELISA Kit; Vegfr1 ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; VEGFR-1 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FLT-1 ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit
缩写：FLT1
Uniprot No.：P53767
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-110 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.6212.5572.5892.412 502.0051.9461.9761.799 251.2861.3321.3091.132 12.50.8930.8590.8760.699 6.250.5140.5210.5180.341 3.120.3970.4080.4030.226 1.560.2600.2520.2560.079 00.1790.1740.177 | ||||||
货期：3-5 working days
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