首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管内皮细胞生长因子受体1(VEGFR-1/Flt1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFR-1/Flt1含量。VEGFR-1/Flt1 是重要的血管生成相关靶点。它在胚胎发育、肿瘤血管生成等生理病理过程中有重要作用。其与配体结合后激活信号通路，调控内皮细胞增殖、迁移和存活，成为肿瘤等疾病药物研发的潜在靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.39 ng/mL。该产品可应用于心血管疾病模型、肿瘤微环境研究、血管生成调控机制探索等科研领域，支持体外检测小鼠血液或组织样本中VEGFR - 1的动态变化，为血管相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Flt1 ELISA Kit; Emrk2 ELISA Kit; Flt ELISA Kit; Vegfr1 ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; VEGFR-1 ELISA Kit; EC 2.7.10.1 ELISA Kit; Embryonic receptor kinase 2 ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FLT-1 ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit
缩写：FLT1
Uniprot No.：P35969
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 100-106 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-107 | ||||||
回收率：
| The recovery of mouse VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 80-90 | |||||
| EDTA plasma (n=4) | 108 | 104-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 1002.6022.6312.6172.495 502.0032.0682.0361.914 251.3391.3611.3501.228 12.50.7850.7790.7820.660 6.250.4520.4610.4570.335 3.120.2840.2910.2880.166 1.560.1860.1870.1870.065 00.1200.1230.122 | |||||||
货期：3-5 working days
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