首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠丝聚蛋白(FLG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FLG含量。FLG即丝聚蛋白，是皮肤角质形成细胞终末分化产物。它与皮肤屏障功能密切相关，编码基因缺陷可导致皮肤屏障受损、水分丢失增加。其研究机制聚焦于阐释在特应性皮炎等皮肤病中的作用，探寻基于FLG的诊断标记和治疗靶点。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域探索小鼠模型中FLG的表达调控机制，如研究皮肤屏障功能障碍、特应性皮炎等疾病的分子机制，或评估药物干预、基因修饰对FLG蛋白水平的影响。为体外实验或动物模型研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FlgFilaggrin ELISA Kit; Fragment ELISA Kit
缩写：FLG
Uniprot No.：P11088
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 86-95 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-95 | |||||
回收率：
| The recovery of mouse FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 101 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.2902.4332.3622.1971001.5031.3901.4471.282500.8100.8710.8410.676250.5730.6100.5920.42712.50.3810.3660.3740.2096.250.2880.2740.2810.1163.120.2290.2390.2340.06900.1630.1670.165 | ||||||
货期：3-5 working days
×
