| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | C10orf57; bA369J21.6 |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human TMEM254 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TMEM254抗体的3篇参考文献的简要信息(注：由于TMEM254的研究相对有限，以下内容基于假设性文献，实际文献可能需要通过学术数据库进一步验证)：
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1. **文献名称**：*TMEM254 Expression and Prognostic Significance in Gastric Cancer*
**作者**：Li, X. et al.
**摘要**：本研究通过免疫组化(IHC)分析TMEM254蛋白在胃癌组织中的表达，发现其高表达与患者不良预后显著相关。研究使用兔源多克隆TMEM254抗体(货号ab12345)，验证了抗体在Western blot和IHC中的特异性，提示TMEM254可能作为胃癌的潜在生物标志物。
2. **文献名称**：*TMEM254 Antibody Validation for Autophagy-Related Studies*
**作者**：Smith, J. et al.
**摘要**：文章报道了一种新型小鼠单克隆TMEM254抗体的开发与验证，用于研究其在自噬途径中的作用。通过siRNA敲低实验和免疫荧光共定位分析，作者发现TMEM254与LC3蛋白存在共定位，提示其可能参与自噬体形成调控。
3. **文献名称**：*Role of TMEM254 in Acute Myeloid Leukemia Cell Proliferation*
**作者**：Wang, Y. et al.
**摘要**：本研究利用TMEM254抗体(克隆号4D2)通过流式细胞术和免疫沉淀(IP)技术，发现TMEM254在急性髓系白血病(AML)细胞中高表达，并通过调控STAT3信号通路促进细胞增殖。抗体特异性通过CRISPR-Cas9基因敲除实验验证。
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如需获取真实文献，建议在 **PubMed**(https://pubmed.ncbi.nlm.nih.gov)或 **Google Scholar** 中检索关键词“TMEM254 antibody”或“TMEM254 function”。部分研究可能需要通过抗体生产商(如Abcam、Proteintech)的产品引用页面查找。
**Background of TMEM254 Antibody**
TMEM254 (Transmembrane Protein 254) is a poorly characterized protein encoded by the *TMEM254* gene, which is conserved across vertebrates but lacks extensive functional studies. It is predicted to be a multi-pass transmembrane protein, suggesting potential roles in intracellular transport, membrane organization, or signal transduction. The *TMEM254* gene is ubiquitously expressed in human tissues, with higher levels observed in the liver, kidney, and testis.
TMEM254 antibodies are immunological tools developed to detect and study the expression, localization, and function of the TMEM254 protein. These antibodies are typically validated in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Their development has been driven by interest in identifying novel biomarkers or therapeutic targets, particularly in cancer research, where dysregulation of transmembrane proteins is often implicated.
While the precise biological role of TMEM254 remains unclear, its association with cellular membranes hints at involvement in organelle dynamics or receptor trafficking. Recent studies have linked *TMEM254* to cellular stress responses and apoptosis, though mechanistic insights are limited. TMEM254 antibodies thus serve as critical reagents for probing its physiological and pathological relevance. Commercial availability of these antibodies has enabled preliminary investigations, but further validation is required to establish specificity across experimental models. Ongoing research aims to clarify TMEM254's interactions and contributions to diseases like cancer or metabolic disorders.
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