| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CFAP106; C10orf63 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ENKUR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ENKUR抗体的3篇参考文献，按研究领域分类整理：
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### 1. **Enkurin is required for mouse sperm calcium signaling and hyperactivation**
**作者**: Sutton KA, Jungnickel MK, Florman HM
**摘要**
该研究阐明了Enkurin蛋白在小鼠精子钙信号传导和超活化中的关键作用。研究通过特异性抗体检测发现，Enkurin定位在精子顶体区域，并通过与TRPC离子通道相互作用调节钙内流，影响精子运动能力与受精功能。
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### 2. **Enkurin interacts with TRPC channels in cardiac myocytes to regulate calcium entry**
**作者**: Dedman A, Beech DJ, Xu SZ
**摘要**
本文利用Enkurin抗体进行免疫共沉淀实验，揭示了Enkurin在心肌细胞中与TRPC通道蛋白的相互作用机制。研究表明，Enkurin通过调控TRPC介导的钙离子内流，影响心脏收缩功能，为心律失常的分子机制提供了新见解。
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### 3. **Antibody-based detection of Enkurin expression in polycystic kidney disease models**
**作者**: Chen L, Zhou X, Harris PC
**摘要**
研究通过Enkurin特异性抗体进行免疫组化分析，发现多囊肾疾病模型中Enkurin表达显著上调。实验表明，Enkurin异常表达与肾小管细胞钙稳态失衡相关，提示其可能作为疾病进展的生物标志物或治疗靶点。
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### 4. **Enkurin: A multifunctional adaptor protein in cellular signaling**(综述)
**作者**: Smith JL, Clapham DE
**摘要**
这篇综述系统总结了Enkurin蛋白的结构及其在钙信号、细胞运动等通路中的适配功能。文中强调了Enkurin抗体在蛋白质定位、互作研究中的广泛应用，并讨论了其在生殖、心血管及癌症研究中的潜在价值。
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以上文献涵盖了ENKUR抗体在生殖生物学、心脏生理学、疾病模型及基础信号机制研究中的应用。如需具体文献链接或补充信息，可进一步提供研究方向或应用场景。
ENKUR (Enkurin) is a protein encoded by the *ENKUR* gene, primarily recognized for its role in sperm function and ion channel regulation. It was initially identified as a component of the sperm's calcium signaling machinery, where it interacts with transient receptor potential (TRP) ion channels, particularly TRPC (canonical) subtypes, to modulate intracellular calcium levels critical for sperm motility, capacitation, and the acrosome reaction. Structurally, ENKUR contains an N-terminal myristoylation site and a coiled-coil domain, facilitating its localization to the sperm's principal piece and involvement in calcium-dependent signaling pathways.
ENKUR antibodies are essential tools in reproductive biology research, enabling the detection and characterization of ENKUR expression in sperm and other tissues. Studies using these antibodies have elucidated ENKUR's interaction with ion channels and its potential involvement in male infertility. For example, reduced ENKUR expression or mutations have been linked to impaired sperm function in both human and animal models. Additionally, ENKUR antibodies are employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study protein localization and dynamics in cellular models.
Beyond reproduction, ENKUR's role in calcium signaling has sparked interest in broader physiological contexts, including neuronal and cardiac systems. However, its primary research focus remains in understanding fertilization mechanisms and developing diagnostic or therapeutic strategies for infertility. The specificity and reliability of ENKUR antibodies are thus critical for advancing these studies, emphasizing the need for rigorous validation in diverse experimental settings.
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