| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | APC15; HSPC020; C11orf51 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ANAPC15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ANAPC15抗体的3篇参考文献示例(注：以下内容为模拟生成，部分文献可能不存在或需替换为真实文献)：
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1. **文献名称**：*"APC15 regulates mitotic exit through its role in APC/C-mediated substrate processing"*
**作者**：Zhang, S., et al.
**摘要**：本研究阐明了ANAPC15(APC15)在APC/C复合体调控有丝分裂退出中的机制。通过使用特异性ANAPC15抗体进行免疫沉淀和蛋白质质谱分析，发现其介导APC/C底物识别及泛素化降解，敲低ANAPC15会导致细胞周期阻滞。
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2. **文献名称**：*"Structural insights into the APC/C-ANAPC15 interaction and its implications for cancer"*
**作者**：Li, Y., & Wang, H.
**摘要**：该研究通过冷冻电镜解析了APC/C复合体中ANAPC15的结构，并利用ANAPC15抗体验证其在多种癌细胞系中的表达水平。结果显示ANAPC15过表达与结直肠癌进展相关，提示其作为潜在治疗靶点。
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3. **文献名称**：*"A functional screen identifies ANAPC15 as a regulator of neuronal differentiation"*
**作者**：Chen, L., et al.
**摘要**：文章发现ANAPC15通过调控APC/C活性影响神经干细胞分化。使用ANAPC15抗体进行免疫荧光和Western blot分析，证实其敲除导致分化标志物表达异常，提示其在神经发育中的关键作用。
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如需获取真实文献，建议在PubMed或Google Scholar检索关键词“ANAPC15 antibody”或“APC15 antibody”，并筛选实验方法学中明确使用该抗体的研究。
The ANAPC15 antibody targets the Anaphase-Promoting Complex/Cyclosome (APC/C) subunit 15. a critical component of the ubiquitin-proteasome system regulating cell cycle progression. The APC/C is a multi-subunit E3 ubiquitin ligase essential for mitotic exit and the transition between cell cycle phases by marking specific substrates (e.g., cyclins, securins) for proteasomal degradation. ANAPC15. a conserved core subunit, contributes to APC/C assembly and substrate recognition, interacting with other subunits like APC2 and APC8 (CDC23).
Antibodies against ANAPC15 are valuable tools for studying APC/C structure, function, and regulation in cell cycle control, DNA damage response, and developmental processes. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess APC/C localization, activity, or expression changes under experimental conditions (e.g., drug treatments, gene knockdown). Dysregulation of APC/C components, including ANAPC15. is implicated in cancers, neurodegenerative disorders, and developmental defects, making these antibodies relevant for both basic research and therapeutic exploration. Commercial ANAPC15 antibodies are typically raised in rabbits or mice, validated for specificity via knockout/knockdown controls. Studies using these antibodies have helped clarify mechanisms of APC/C-mediated ubiquitination and its role in maintaining genomic stability.
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