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产品描述：牛纤连蛋白(FN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的FN1含量。FN1即纤连蛋白 1，是细胞外基质的重要成分，在细胞黏附、迁移、增殖等过程中发挥关键作用。研究显示，其异常表达与肿瘤的发生、发展及转移密切相关。深入探究 FN1 的作用机制，有望为肿瘤等疾病的治疗提供新靶点和思路。试剂盒检测范围为0.137 μg/mL-100 μg/mL，科研人员可将其应用于细胞外基质功能研究、细胞粘附机制探索、组织再生模型构建或炎症相关疾病模型的分子机制分析，例如评估体外培养细胞分泌FN的水平，或分析特定病理状态下组织微环境中FN的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FN1Fibronectin ELISA Kit; FN) [Cleaved into: Anastellin] ELISA Kit
缩写：FN1
Uniprot No.：P07589
种属：Bos taurus (Bovine)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.137 μg/mL-100 μg/mL
灵敏度：0.137 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 92 | |||||
| Range % | 85-102 | ||||||
| 1:10 | Average % | 97 | |||||
| Range % | 91-110 | ||||||
| 1:20 | Average % | 89 | |||||
| Range % | 83-96 | ||||||
| 1:40 | Average % | 95 | |||||
| Range % | 84-100 | ||||||
回收率：
| The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 88-105 | |||||
| EDTA plasma (n=4) | 97 | 92-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| µg/mlOD1OD2AverageCorrected 1001.8391.8031.8211.677 331.6571.6491.6531.509 111.4511.4351.4431.299 3.70.9580.9920.9750.831 1.20.6430.6670.6550.511 0.40.3720.3540.3630.219 0.130.2510.2430.2470.103 00.1450.1420.144 | |||||||
货期：3-5 working days
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