首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人甘丙肽样肽(GALP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GALP含量。GALP（甘丙肽样肽）是一种神经肽。其背景上，它在中枢神经系统等有表达。研究机制方面，GALP与甘丙肽受体相互作用，参与调节能量代谢、生殖功能、摄食行为等生理过程，在生物体内发挥着重要的神经调节作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，为体外实验研究其生理病理机制提供可靠工具，尤其适用于探究代谢调控、神经内分泌网络及相关疾病模型的分子机制，满足基础科研中对GALP表达水平动态监测的需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GALP ELISA Kit; Galanin-like peptide ELISA Kit
缩写：GALP
Uniprot No.：Q9UBC7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-102 | ||||
| EDTA plasma (n=4) | 94 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.0572.0802.0691.91851.7611.8121.7871.6362.51.4031.3731.3881.2371.250.9880.9730.9810.8300.6250.7030.7270.7150.5640.3120.4770.4970.4870.3360.1560.3220.3310.3270.17600.1500.1520.151 | ||||||
货期：3-5 working days
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