首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠甘丙肽样肽(GALP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GALP含量。GALP（甘丙肽样肽）是一种神经肽，其基因编码的蛋白质与甘丙肽受体1、2和3结合，对受体3亲和力最高，涉及中枢神经系统生物过程，包括下丘脑对代谢和繁殖的调节。研究显示，GALP与多种代谢和内分泌指标密切相关，可能在多囊卵巢综合征（PCOS）等疾病中发挥重要作用。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，本试剂盒适用于探索其在肥胖、糖尿病等代谢性疾病模型中的表达变化，或用于神经内分泌调控机制的基础研究，为体外定量分析GALP提供高特异性的科研工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GalpGalanin-like peptide ELISA Kit
缩写：GALP
Uniprot No.：Q810H5
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 86-97 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 87-95 | ||||||
回收率：
| The recovery of mouse GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 83-92 | |||||
| EDTA plasma (n=4) | 94 | 87-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.4212.5362.4792.2887501.9052.0111.9581.7673751.3281.4131.3711.180187.50.8210.8340.8280.637940.5140.5270.5210.330470.3700.3870.3790.18823.50.3110.3150.3130.12200.1900.1920.191 | |||||||
货期：3-5 working days
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