| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NIMP; OPA10 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RTN4IP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RTN4IP1抗体的3篇模拟参考文献(基于领域知识合理构造，非真实存在)：
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1. **文献名称**：RTN4IP1 Antibody Validation for Mitochondrial Localization Studies
**作者**：Lee S, et al.
**摘要**：本研究通过Western blot和免疫荧光技术验证了RTN4IP1抗体特异性，证明其可识别内源性RTN4IP1蛋白，并发现该蛋白定位于线粒体外膜，与氧化磷酸化复合物的组装相关。
2. **文献名称**：Role of RTN4IP1 in Retinal Ganglion Cell Degeneration
**作者**：Chen J, et al.
**摘要**：利用RTN4IP1抗体进行小鼠视网膜组织染色，发现该蛋白在视网膜神经节细胞中高表达;基因敲除模型显示RTN4IP1缺失导致线粒体功能障碍和细胞凋亡，提示其与青光眼相关神经退变有关。
3. **文献名称**：RTN4IP1 Interaction Network Revealed by Immunoprecipitation
**作者**：Müller T, et al.
**摘要**：通过RTN4IP1抗体进行免疫共沉淀联合质谱分析，鉴定出多个线粒体代谢相关蛋白互作伴侣，包括复合物I亚基NDUFS1.支持其在能量代谢中的调控作用。
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注：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以“RTN4IP1 antibody”为关键词检索真实文献。
The RTN4IP1 antibody targets the reticulon 4-interacting protein 1 (RTN4IP1), a mitochondrial enzyme implicated in neuronal development and metabolic regulation. RTN4IP1. also known as neuroendocrine regulatory protein 2 (NERP2), interacts with reticulon 4 (RTN4/NOGO) to modulate mitochondrial function, oxidative phosphorylation, and neuroaxonal growth. It belongs to the NADH dehydrogenase (ubiquinone) complex and plays a role in maintaining mitochondrial integrity, energy production, and reactive oxygen species (ROS) homeostasis. Dysregulation of RTN4IP1 is associated with neurological disorders, including optic atrophy, epilepsy, and neurodevelopmental defects.
Antibodies against RTN4IP1 are primarily used in research to investigate its expression, localization, and mechanistic roles in cellular and animal models. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding studies on mitochondrial dynamics, neuronal connectivity, and disease pathology. Recent studies highlight RTN4IP1's involvement in zebrafish visual system development and mammalian brain function, emphasizing its conservation across species. Researchers also explore its potential as a biomarker or therapeutic target for mitochondrial encephalopathies. Commercial RTN4IP1 antibodies are typically validated for specificity in human, mouse, or rat tissues, supporting translational research in neurodegeneration and metabolic syndromes.
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