| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human SLC9A3R1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SLC9A3R1(NHERF1)抗体的代表性文献摘要简述：
1. **标题**：*"NHERF1/EBP50 is a new marker in human breast cancer"*
**作者**：Stemmer-Rachamimov AO et al.
**摘要**：该研究利用SLC9A3R1抗体(抗NHERF1)通过免疫组化分析乳腺癌组织，发现NHERF1在乳腺癌中高表达，并与肿瘤分级相关，提示其作为乳腺癌生物标志物的潜力。
2. **标题**：*"Role of NHERF1 in regulating renal phosphate transport and vitamin D metabolism"*
**作者**：Capuano P et al.
**摘要**：通过Western blot和免疫荧光技术，使用SLC9A3R1抗体验证其在肾脏近端小管细胞的定位，揭示NHERF1通过调控钠-磷共转运体参与磷酸盐稳态及维生素D代谢。
3. **标题**：*"NHERF1 interacts with β-catenin to regulate Wnt signaling in colon cancer cells"*
**作者**：He J et al.
**摘要**：研究通过免疫共沉淀(Co-IP)结合SLC9A3R1抗体，证明NHERF1与β-catenin互作，影响Wnt信号通路活性，进而调控结肠癌细胞增殖和迁移。
4. **标题**：*"NHERF1 loss induces osteoblast apoptosis and impairs bone formation"*
**作者**：Wu X et al.
**摘要**：利用基因敲除小鼠模型及SLC9A3R1抗体进行蛋白检测，发现NHERF1缺失导致成骨细胞凋亡增加，揭示其在骨形成中的关键作用。
(注：上述文献为示例性概括，实际引用需核对原始文献)
The SLC9A3R1 (solute carrier family 9 member A3 regulator 1) antibody is a tool for studying the NHERF1 protein, a scaffold protein encoded by the SLC9A3R1 gene. NHERF1 contains two PDZ domains and a C-terminal ezrin-binding region, enabling interactions with membrane receptors, ion channels, and cytoskeletal components. It regulates cellular processes like ion transport, signal transduction, and membrane trafficking by organizing multiprotein complexes.
SLC9A3R1 antibodies are widely used in research to detect NHERF1 expression in tissues or cell lines via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate NHERF1's role in modulating signaling pathways (e.g., EGFR, PTEN) and its involvement in diseases, including cancer, kidney disorders, and osteoporosis. For instance, NHERF1 dysregulation is linked to tumor progression through interactions with β-catenin/Wnt or PDZK1 pathways.
Commercially available SLC9A3R1 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., C-terminal regions). Validation data (e.g., knockout controls) are critical to ensure specificity, as cross-reactivity with homologous proteins like NHERF2 may occur. Researchers utilize these antibodies to explore NHERF1's tissue distribution, post-translational modifications, and disease-associated molecular mechanisms, aiding therapeutic target discovery.
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