| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L1.2; LRE1 |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human L1RE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于L1RE1抗体的参考文献示例(注：部分信息为模拟生成，实际文献需通过学术数据库查询确认)：
1. **文献名称**：*LINE-1 ORF1p Expression in Human Cancers: A Retrotransposon-Driven Oncogenic Mechanism?*
**作者**：Burns, K.H. et al.
**摘要**：本研究利用L1RE1抗体检测多种肿瘤组织中LINE-1 ORF1蛋白的表达，发现其在肺癌和结肠癌中显著上调，提示转座子活化可能通过诱导基因组不稳定促进肿瘤发生。
2. **文献名称**：*Immunolocalization of L1 Retrotransposon-Encoded Proteins in Human Germ Cells*
**作者**：Garcia-Perez, J.L. et al.
**摘要**：通过L1RE1抗体进行免疫荧光分析，揭示LINE-1 ORF1蛋白在人类精母细胞中的特异性表达，支持其在生殖细胞发育过程中可能的表观遗传调控作用。
3. **文献名称**：*L1RE1 Antibody-Based Detection of Retrotransposon Activity in Neurodegenerative Disorders*
**作者**：Muotri, A.R. et al.
**摘要**：应用L1RE1抗体在阿尔茨海默病患者脑组织中观察到LINE-1 ORF1蛋白的异常聚集，表明转座子激活可能与神经炎症及神经元退行性变相关。
4. **文献名称**：*Characterization of a Monoclonal Antibody Targeting LINE-1 ORF1p for Functional Studies*
**作者**：Goodier, J.L. et al.
**摘要**：本文详细描述了L1RE1单克隆抗体的开发与验证，证实其特异性识别LINE-1 ORF1蛋白，并成功应用于体外逆转录酶活性抑制实验及小鼠模型中的转座子追踪。
**提示**：以上为模拟文献，实际研究中建议通过PubMed或Google Scholar以关键词“L1RE1 antibody”、“LINE-1 ORF1”等检索最新文献，并关注领域内权威团队(如Kazazian, Burns, Muotri等)的研究。
The L1RE1 antibody targets the LINE-1 (L1) retrotransposon-encoded open reading frame 1 (ORF1) protein, a key component of autonomous L1 elements. LINE-1 retrotransposons constitute ~17% of the human genome and are capable of replicating via a "copy-and-paste" mechanism, contributing to genomic diversity, evolution, and instability. The ORF1 protein, a nucleic acid chaperone, facilitates L1 RNA packaging and reverse transcription during retrotransposition. Dysregulated L1 activity is linked to cancer, neurological disorders, and autoimmune diseases, making ORF1 a biomarker for active retrotransposition.
The L1RE1 monoclonal antibody, often raised in mice, is widely used to detect ORF1 in immunoassays (e.g., Western blot, immunohistochemistry) and to study L1 biology in developmental, aging, and disease contexts. It helps elucidate mechanisms of retrotransposition suppression (e.g., by DNA methylation or antiviral pathways) and pathological consequences of L1 reactivation. Research using L1RE1 has highlighted L1's dual role as both a driver of genetic innovation and a potential threat to genomic integrity, offering insights into therapeutic strategies targeting retrotransposon-related pathologies.
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