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产品描述：猪神经胶质纤维酸性蛋白(GFAP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GFAP含量。GFAP（胶质纤维酸性蛋白）是一种特定于中枢神经系统的III类中间丝蛋白，主要在星形胶质细胞中表达。其研究机制涉及基因表达调控、蛋白结构、功能以及与多种神经系统疾病的关系，如阿尔茨海默病和亚历山大病。GFAP在维持星形胶质细胞形态、血脑屏障形成和突触功能中发挥重要作用。试剂盒检测范围为0.1 ng/mL-20 ng/mL，本试剂盒适用于猪模型在神经退行性疾病研究（如阿尔茨海默病、帕金森病）、脑创伤模型评估、神经毒性实验或神经炎症机制探索等科研场景中，定量分析血液或组织样本的GFAP动态变化，为神经病理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GFAP
Uniprot No.：F1RR02
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.1 ng/mL-20 ng/mL
灵敏度：0.05 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of pig GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-105 | ||||
| EDTA plasma (n=4) | 96 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.1870.1950.191 50.3380.3460.342 1.60.5590.5510.555 0.40.8420.8680.855 0.11.3341.3371.336 01.9892.0051.997 | ||||||
货期：3-5 working days
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